Mastrobuoni will start at shortstop and bat sixth in Wednesday's game against Atlanta, Maddie Lee of the Chicago Sun-Times reports.
Mastrobuoni went 3-for-4 with a walk, a double, an RBI and two runs scored in Saturday's 10-9 loss to the Pirates.
The Cubs are expected to call up Mastrobuoni from Triple-A Iowa before Saturday's game against the Brewers, Patrick Mooney of The Athletic reports.
The Cubs optioned Mastrobuoni to Triple-A Iowa on Thursday, Taylor McGregor of Marquee Sports Network reports.
The Cubs informed Mastrobuoni on Monday that he'll be included on the Opening Day roster, Meghan Montemurro of the Chicago Tribune reports.
Mastrobuoni seems likely to secure one of Chicago's final bench spots coming out of camp with Nick Madrigal (hamstring) possibly set to miss time, Jordan Bastian of MLB.com reports.
Mastrobuoni went 2-for-3 with a walk and two runs scored in Saturday's win over the Rockies. He also stole a base.
The Cubs recalled Mastrobuoni from Triple-A Iowa on Saturday, Andy Martinez of Marquee Sports Network reports.
Mastrobuoni was optioned to Triple-A Iowa on Saturday, Meghan Montemurro of the Chicago Tribune reports.
Mastrobuoni isn't in the Cubs' lineup Friday against the Cardinals.