Rosario was optioned to Triple-A El Paso on Sunday.
Rosario has started at third base in three of San Diego's past four games, with Thursday's start being particularly notable since it came with the Padres facing a right-handed hurler in Colorado's Dakota Hudson.
Rosario will start at third base and bat seventh in Wednesday's game against the Cardinals.
Rosario went 1-for-2 with a two-run home run in Saturday's loss to the Giants.
Rosario went 1-for-4 with a solo home run in Sunday's loss to Houston.
Rosario was recalled from Triple-A El Paso on Friday.
Rosario is expected to be promoted to the Padres' major-league roster Friday, Kevin Acee of the San Diego Union-Tribune reports.
The Padres reinstated Rosario (ankle) from the 60-day injured list Friday and optioned him to Triple-A El Paso.
Rosario (ankle) resumed his rehab assignment at Triple-A El Paso on Tuesday and went 1-for-3 with a home run, a walk and two additional RBI while playing nine innings at second base in a 5-2 win over Round Rock.
Rosario (ankle) resumed his rehab assignment June 22 and has gone 4-for-17 with six RBI, three runs and a 4:6 BB:K through five games with Triple-A El Paso this season.